Temperature-sensitive revertants
نویسندگان
چکیده
منابع مشابه
Temperature-sensitive pleiotropic revertants from a mutant in the arom gene cluster of Neurospora crassa.
Genetical and biochemical studies have been performed with revertants induced in a polyaromatic mutant (No. 58) in the arom gene cluster of Neurospora crassa. In addition to complete and partial revertants able to grow on minimal at both 25 degrees and 35 degrees , temperature-sensitive revertants capable of growth on minimal at 25 degrees but not at 35 degrees have been recovered. One of these...
متن کاملCharacterization of a temperature-sensitive Escherichia coli mutant and revertants with altered seryl-tRNA synthetase activity.
A mutation in the structural gene coding for seryl-tRNA synthetase in temperature-sensitive Escherichia coli K28 has been reported to alter the level of enzyme expression at high temperature (R. J. Hill and W. Konigsberg, J. Bacteriol. 141:1163-1169, 1980). We identified this mutation as a C-->T transition in the first base of codon 386, resulting in a replacement of histidine by tyrosine. The ...
متن کاملTemperature-sensitive mutant vaccines.
Many live virus vaccines derived by empirical routes exhibit temperature-sensitive (ts) phenotypes. The live virus vaccines that have been outstandingly successful in controlling poliomyelitis are the prime example of this phenomenon. The three live attenuated strains developed by Sabin were derived from wild-type isolates by rapid sequential passage at high multiplicity of infection in monkey ...
متن کاملTemperature-sensitive mutants and revertants in the coronavirus nonstructural protein 5 protease (3CLpro) define residues involved in long-distance communication and regulation of protease activity.
Positive-strand RNA virus genomes are translated into polyproteins that are processed by viral proteases to yield functional intermediate and mature proteins. Coronaviruses (CoVs) carry genes that encode an nsp5 protease (also known as 3CLpro or Mpro) responsible for 11 maturation cleavages. The nsp5 structure contains two chymotrypsin-like domains (D1 and D2) and a unique domain (D3), and form...
متن کاملTemperature-sensitive protein-DNA dimerizers.
Programmable DNA-binding polyamides coupled to short peptides have led to the creation of synthetic artificial transcription factors. A hairpin polyamide-YPWM tetrapeptide conjugate facilitates the binding of a natural transcription factor Exd to an adjacent DNA site. Such small molecules function as protein-DNA dimerizers that stabilize complexes at composite DNA binding sites. Here we investi...
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ژورنال
عنوان ژورنال: Biochemical and Biophysical Research Communications
سال: 1971
ISSN: 0006-291X
DOI: 10.1016/0006-291x(71)90166-5